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Mohan Krishna Lokireddy ¹, P. Jayachandra Reddy ², Nallathambi Ramasamy ¹, B. Prabhakar Reddy ¹

Affiliations
1 Vignan Institute of Pharmaceutical Sciences, Deshmukhi, Nalgonda, Andhra Pradesh, IN
2 Krishna Teja Pharmacy College, Tirupati, IN

Abstract

Four simple and sensitive spectrophotometric methods (A - D) for the assay of Aripiprazole in pure and dosage forms based on the formation of chloroform soluble ion-associates under specified experimental conditions are described. Four acidic dyes, namely, wool fast Blue (WFB BL, method A), orange II (Tpooo, method B), Naphthalene Blue 12 BR (NB-12BR, method C), and Azocarmine (AG, method D) are utilized. The extracts of the ion-associates exhibit absorption maxima at 580, 480, 630 and 540 nm for methods A, B, C and D, respectively. Beer's law and the precision and accuracy of the methods are checked by the UV reference.

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Mohan Krishna Lokireddy ¹, P. Jayachandra Reddy ², M. Srinivasa Murthy ¹

Affiliations
1 Vignan Institute of Pharmaceutical Sciences, Deshmukhi, Nalgonda, Andhrapradesh, IN
2 Krishna Teja Pharmacy College, Tirupati, IN

Abstract

Two simple and sensitive visible spectrophotometric methods (A and B) have been developed for the determination of Amphotericin B (AMP) in bulk and pharmaceutical formulations. These methods are based on the Method A was developed based on the formation of a colored condensation reaction with p-dimethyl amino cinnamaldehyde (PDAC) under acidic conditions (λmax 490 nm), and method B based on the oxidative coupling reaction with DCQC (2,6-dichloroquinone N-chlorimide) (λmax 530nm) . Beer's law limits, precision and accuracy of these methods are checked by the UV reference method. The results obtained are reproducible and are statistically validated and so found to be suitable for the assay of in bulk and pharmaceutical formulations.

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